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Objective To understand the etiological characteristics and risk factors of respiratory virus infection in children with bronchial asthma, and to provide theoretical basis for the prevention and treatment of respiratory virus infection in children with bronchial asthma. Methods A total of 374 children with bronchial asthma who were treated in Jianyang People's Hospital from December 2018 to December 2020 were enrolled. Pharyngeal swabs were collected from the outpatient children on the day of treatment, and 2 mL of nasopharyngeal secretions were collected from the hospitalized children within 24 hours by negative pressure aspirator. Seven viral antigens including RSV, ADV, IVA, IVB, PIVI, PIV II, and PIV III were detected. According to whether the virus test results were positive or not, they were divided into the experimental group (n=191) and the control group (n=183). Logistic regression analysis was used to screen the risk factors of respiratory virus infection in children with bronchial asthma. Results Among the 374 samples, the virus positive rate was 51.07% (191/374), and the top 3 virus species in the positive samples were RSV, ADV, and PIV III, accounting for 41.36% (79/191), 30.36% (58/191), and 9.42% (18/191), respectively. In addition, IVA accounted for 5.24% (10/191), PIV II accounted for 5.24% (10/191), PIVI accounted for 3.66% (7/191), and IVB accounted for 1.57% (3 /191). The positive rates of virus were 47.96% (94/196) and 54.49% (97/178) in male and female children, respectively, with no significant difference (χ2=1.597，P>0.05). The positive rate of 1~3 years old children was significantly higher than that of >3 years old group (χ2=6.412，P3 times, intravenous glucocorticoid application and onset season were independent risk factors for respiratory virus infection in children with bronchial asthma (P<0.05). Conclusion The infection season of acute respiratory tract infection in children with asthma is mainly concentrated in autumn and winter, with RSV as the main viral pathogen. Targeted preventive measures should be given to children with bronchial asthma who have more than 3 asthma attacks and intravenous glucocorticoid application, which can reduce respiratory virus infection in children with asthma.
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<p><b>OBJECTIVE</b>To investigate the epidemiological characteristics of hand-foot-mouth disease.</p><p><b>METHODS</b>A total of 589 children from Xianju county, Zhejiang province with hand-foot-mouth disease in 2013 were recruited in the study and the diagnosis went through clinical and laboratory confirmation. Attributes that were analyzed included gender, age, month of occurrence, regional distribution and clinical presentation.</p><p><b>RESULTS</b>The incidence rate of hand-foot-mouth disease in Xianju county for the year was 102.72/100 000, with 12 severe cases but no death. There were clearly more boys than girls, with a male to female ratio of 1.29 :1, which was statistically significant (χ(2) = 14.274, P < 0.001). The vast majority of patients were 0-3 years old, representing 94.74% of the patients. The occurrences were concentrated in the period from April to August and November, with April as the high-incidence season. High incidence areas were the countryside and suburbs, making up 45.50% and 30.73%, respectively, of all cases. Most patients were in preschools or scattered home care environments, accounting for 51.10% and 40.92% , respectively. Clinical presentation was characterized by fever and various kinds of vesicles or papules at the hand, foot, mouth or buttocks.</p><p><b>CONCLUSION</b>Most of hand-foot-mouth disease cases occur in younger children in areas with inadequate knowledge of hygiene, with seasonal variation.</p>
Subject(s)
Child , Child, Preschool , Female , Humans , Infant , Male , China , Epidemiology , Hand, Foot and Mouth Disease , EpidemiologyABSTRACT
Objective To investigate the epidemiological characteristics of hand-foot-mouth disease. Methods A total of 589 children from Xianju county,Zhejiang province with hand-foot-mouth disease in 2013 were recruited in the study and the diagnosis went through clinical and laboratory confirmation. Attributes that were analyzed included gender,age,month of occurrence, regional distribution and clinical presentation. Results The incidence rate of hand-foot-mouth disease in Xianju county for the year was 102.72/100 000,with 12 severe cases but no death. There were clearly more boys than girls,with a male to female ratio of 1.29∶1,which was statistically significant(χ2=14.274,P<0.001). The vast majority of patients were 0-3 years old,representing 94.74% of the patients. The occurrences were concentrated in the period from April to August and November,with April as the high-incidence season. High incidence areas were the countryside and suburbs,making up 45.50%and 30.73%,respectively,of all cases. Most patients were in preschools or scattered home care environments,accounting for 51.10% and 40.92% ,respectively. Clinical presentation was characterized by fever and various kinds of vesicles or papules at the hand,foot, mouth or buttocks. Conclusion Most of hand-foot-mouth disease cases occur in younger children in areas with inadequate knowledge of hygiene,with seasonal variation.
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Objective To investigate the epidemiological characteristics of hand-foot-mouth disease. Methods A total of 589 children from Xianju county,Zhejiang province with hand-foot-mouth disease in 2013 were recruited in the study and the diagnosis went through clinical and laboratory confirmation. Attributes that were analyzed included gender,age,month of occurrence, regional distribution and clinical presentation. Results The incidence rate of hand-foot-mouth disease in Xianju county for the year was 102.72/100 000,with 12 severe cases but no death. There were clearly more boys than girls,with a male to female ratio of 1.29∶1,which was statistically significant(χ2=14.274,P<0.001). The vast majority of patients were 0-3 years old,representing 94.74% of the patients. The occurrences were concentrated in the period from April to August and November,with April as the high-incidence season. High incidence areas were the countryside and suburbs,making up 45.50%and 30.73%,respectively,of all cases. Most patients were in preschools or scattered home care environments,accounting for 51.10% and 40.92% ,respectively. Clinical presentation was characterized by fever and various kinds of vesicles or papules at the hand,foot, mouth or buttocks. Conclusion Most of hand-foot-mouth disease cases occur in younger children in areas with inadequate knowledge of hygiene,with seasonal variation.
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<p><b>OBJECTIVE</b>To evaluate the coronal microleakage of teeth restored with fiber posts and composite cores with four different resin bonding systems.</p><p><b>METHODS</b>Sixty extracted human maxillary single-rooted teeth were endodontically treated and randomly divided into four groups. Post spaces were prepared in the root canals. Four different resin bonding systems (RelyX Unicem, Dulink, DC core Automix, Luxacore) were used respectively to bond fiber posts and reconstruct cores. The specimens were immersed in India ink for one week. Subsequently all teeth were demineralized, dehydrated and rendered transparent. Then microleakage was observed with stereomicroscope.</p><p><b>RESULTS</b>The microleakage degrees among four resin bonding systems were significantly different (P = 0.007), and group C showed the least microleakage compared with the other three.</p><p><b>CONCLUSION</b>Self-etching resin bonding systems have a super margin sealing ability.</p>
Subject(s)
Humans , Composite Resins , Dental Bonding , Dental Leakage , Dentin-Bonding Agents , Post and Core Technique , Resin CementsABSTRACT
Objective To discuss the features of nosocomial infection in patients with multiple trauma in intensive care unit and the preventive measures. Method The nosocomial infections occurred in 137 patients with multiple trauma were analyzed including site of infection, pathogenic organisms and bacterial strains. Results The rate of nosocomial infection was 62.8%, mainly the lower respiratory tract infection and wound infection, the G-negative bacteria infection accounted for 67.8%, mostly Escherichia Coli, Pseudomonas aeruginosa and A cinctobacter baumanni I. The G-positive bacteria infection accounted for 32.2%, mainly Staphylococcus aureus and Coagulase negative staphylococcus. The positive detection rates of Klebsiella and pseudomonas aeruginosa were increasing year afer year. Conclusions The incidence of nosocomial infection in patients with multiple trauma is high, suggesting some appropriate preventive measures should be taken to reduce the rate of infection.
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Objective To investigate the inhibiting effects of shRNA(short hairpin RNA,shRNA) on COX-2 gene expression,the cell cycle and growth of gastric carcinoma SGC-7901 cells.Methods Specific shRNA plasmid to COX-2 were constructed,and then transfected into SGC-7901 cells by lipofectamine methods.Tests were divided into three groups: untransfected gastric carcinoma SGC-7901 cells group,negative control HK group and pshRNA-COX-2 group.Gastric carcinoma SGC-7901 cells were transfected with LipofectamineTM 2000.Expression of COX-2mRNA and protein were detected with reverse transcriptional polymerase chain reaction(RT-PCR) and Western blot respectively.Cell cycle analysis and cell growth chart were detected with flow cytometry and cell count respectively.Results Compared with negative control HK group,recombinant expression vector pshRNA-COX-2 resulted in the reduction of COX-2mRNA and protein expresion by 70.1% and 43.2% respectively;cells in G0-G1 phase increased from 61.5%to 70.2%,cells in S phase decreased from 27.3% to 21.7%,and the growth of SGC-7901 cells cells was slowed significantly.Conclusions Recombinant expression vector pshRNA-COX-2 can significantly inhibit the expression of COX-2 gene,result in the increase of cells in G0-G1 phase and decrease of cells in S phase,and suppress proliferation of gastric carcinoma SGC-7901 cells.